Discovery Proteomics
Unlock the full potential of your biological samples with our state-of-the-art Discovery Proteomics services.
Unlock Deep Protein Discovery for Novel Biomarker Identification
At our site in the Netherlands, we provide comprehensive discovery proteomics workflows designed to identify novel protein biomarkers and drug target candidates with exceptional depth and precision.
Supported biological matrices include:
Plasma
Serum
Tissue
Other biological matrices
Find Every Relevant Protein, Even the Rare Ones
Next to standard sample preparation methods, we also apply advanced nanoparticle-based enrichment methods, enabling the coverage of low-abundant yet highly relevant proteins for unbiased biomarker discovery.
Biological Matrices
Plasma, serum, CSF, cell culture and tissue, across human, rat, mouse, dog and many more species.
Particle-Based Enrichment
Automated nanoparticle enrichment for coverage of low-abundant proteins.
High-Resolution Platform
Evosep One with Bruker timsTOF HT, using ultra-fast TIMS and PASEF acquisition methods.
Proteome Coverage
Protein ID's per matrix, for direct and enriched sample preparation.
Direct sample preparation
~7,500 (Heart, Mouse)
~7,800 (Heart, Human)
ID's based on UniProtKB reviewed (Swissprot) FASTA canonical database for each species, except for Dog, where the reference proteome database was used (UP000805418).
Efficient Workflow
From study design to post-analysis support, every step is set up to control variation and deliver high-quality biomarker candidates.
01
Pre-Study Consultancy
- Focus on desired deliverables
- PTM's and isoforms
- Study design, to assure best results and cost benefits
- Quantity of sample
- High throughput vs more focus on proteome coverage
02
Biological Sample
Plasma, Serum, Tissue
- Multiple species, human, rat, mouse, dog & many more
- Plasma, serum, CSF, cell cultures, tissue biopsies, other biological fluids
03
Sample Preparation
Particle-Based Enrichment
- Neat, depletion and enrichment techniques
- Automated processes, including SEER Proteograph nanoparticle enrichment
- Focus on variation control
04
Analytical Platform
High-Resolution Mass Spectrometry
- Evosep One system to assure sample reproducability between injections
- Bruker timsTOF HT with customized ultra-fast acquisition with TIMS and PASEF methods for enhanced chromatographic resolution, tailored to your needs
05
Data Analysis
Quality Assessment of Biomarker Candidates
- Deep proteome coverage, >7,000 proteins in plasma, based on reviewed Uniprot database
- Robust identification and label-free quantification (LFQ) with Spectronaut
- Differential expression analysis, like KEGG, GO-enrichment
- Variation assessment to identify high quality biomarkers with our in-house algorithm
06
Custom Post-Analysis Support
- Focus on desired deliverables
- PTM's and isoforms
- Study design, to assure best results and cost benefits
- Quantity of sample
- High throughput vs more focus on proteome coverage
Let's Talk About Your Biomarker Strategy.
Whether you're optimizing protein coverage, validating novel targets, or exploring enrichment methods, our team can help you design the right proteomics workflow for your needs.
